Which bacterial spore is used to test Ethylene Oxide sterilization cycles?

The correct choice is Bacillus Atrophaeus, as this bacterial spore is specifically utilized to test Ethylene Oxide (EtO) sterilization cycles. Bacillus Atrophaeus spores are well-known for their resistance to the effects of Ethylene Oxide, making them reliable biological indicators for ensuring that the sterilization process is effective in achieving sufficient microbial kill. The use of these spores allows sterile processing departments to monitor the efficacy of their sterilization cycles and ensure patient safety by confirming that all biological contaminants have been effectively eradicated.

Using Bacillus Stearothermophilus is common for steam sterilization processes due to its thermophilic nature, while Clostridium Sporogenes and Bacillus Subtilis are not relevant for testing Ethylene Oxide sterilization. Clostridium Sporogenes, for instance, is more relevant for anaerobic conditions, and Bacillus Subtilis is not specifically used for this type of sterilization testing. Thus, recognizing the appropriate biological indicators for different sterilization methods is crucial for effective sterilization monitoring.